› 3ftn

X-ray diffraction
2.19Å resolution

Q165E/S254K Double Mutant Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of T. brockii ADH by C. beijerinckii ADH

Released:
DOI: 10.2210/pdb3ftn/pdb
PDB entry 3ftn coloured by chain, front view.
PDB entry 3ftn coloured by chain, side view.
PDB entry 3ftn coloured by chain, top view.
Source organisms:
Primary publication:
Biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms.
Goihberg E, Peretz M, Tel-Or S, Dym O, Shimon L, Frolow F, Burstein Y
Biochemistry 49 1943-53 (2010)
PMID: 20102159

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 352 amino acids
Theoretical weight: 37.85 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P25984 (Residues: 1-7, 166-253, 255-301; Coverage: 41%)
  • Canonical: P14941 (Residues: 8-165, 254-254, 302-352; Coverage: 60%)
Gene name: adh
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand ACT 4 x ACT
Ligand EDO 11 x EDO
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 79.376Å b: 83.003Å c: 119.691Å
α: 90° β: 99.93° γ: 90°
R-values:
R R work R free
0.166 0.165 0.228
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains.
Collas et al. (2012)
1 more