› 3fwl

X-ray diffraction
3.09Å resolution

Crystal Structure of the Full-Length Transglycosylase PBP1b from Escherichia coli

Released:
DOI: 10.2210/pdb3fwl/pdb
PDB entry 3fwl coloured by chain, front view.
PDB entry 3fwl coloured by chain, side view.
PDB entry 3fwl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the membrane-bound bifunctional transglycosylase PBP1b from Escherichia coli.
Sung MT, Lai YT, Huang CY, Chou LY, Shih HW, Cheng WC, Wong CH, Ma C
Proc Natl Acad Sci U S A 106 8824-9 (2009)
PMID: 19458048

Function and Biology Details

Reactions catalysed: 
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 1B Chain: A
Molecule details ›
Chain: A
Length: 751 amino acids
Theoretical weight: 84.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02919 (Residues: 58-804; Coverage: 89%)
Gene names: JW0145, b0149, mrcB, pbpF, ponB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand M0E 1 x M0E
1 modified residue:
Ligand MSE 25 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 63.245Å b: 296.997Å c: 62.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.273
Expression system: Escherichia coli

Quick links

Citations

26 review citations

From the regulation of peptidoglycan synthesis to bacterial growth and morphology.
Typas et al. (2011)
25 more

4 mentions without citation

Crystal structure of the membrane-bound bifunctional transglycosylase PBP1b from Escherichia coli.
Sung et al. (2009)
3 more