› 3g0h

X-ray diffraction
2.7Å resolution

Human dead-box RNA helicase DDX19, in complex with an ATP-analogue and RNA

Released:
DOI: 10.2210/pdb3g0h/pdb
PDB entry 3g0h coloured by chain, front view.
PDB entry 3g0h coloured by chain, side view.
PDB entry 3g0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.
Collins R, Karlberg T, Lehtiö L, Schütz P, van den Berg S, Dahlgren LG, Hammarström M, Weigelt J, Schüler H
J Biol Chem 284 10296-300 (2009)
PMID: 19244245

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193968 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase DDX19B Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 48.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 54-475; Coverage: 88%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains:
Structure domains:
5'-R(P*UP*UP*UP*UP*UP*UP*U)-3' Chain: E
Molecule details ›
Chain: E
Length: 7 nucleotides
Theoretical weight: 2.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 41.57Å b: 81.08Å c: 124.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.275
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
24 more

20 mentions without citation

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)
19 more