› 3g45

X-ray diffraction
2.63Å resolution

Crystal structure of human phosphodiesterase 4b with regulatory domain and d155988

Released:
DOI: 10.2210/pdb3g45/pdb
PDB entry 3g45 coloured by chain, front view.
PDB entry 3g45 coloured by chain, side view.
PDB entry 3g45 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design of phosphodiesterase 4D (PDE4D) allosteric modulators for enhancing cognition with improved safety.
Burgin AB, Magnusson OT, Singh J, Witte P, Staker BL, Bjornsson JM, Thorsteinsdottir M, Hrafnsdottir S, Hagen T, Kiselyov AS, Stewart LJ, Gurney ME
Nat Biotechnol 28 63-70 (2010)
PMID: 20037581

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-AMP phosphodiesterase 4B Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 48.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07343 (Residues: 241-289, 305-659; Coverage: 55%)
Gene names: DPDE4, PDE4B
Sequence domains:
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand 988 2 x 988
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P31
Unit cell:
a: 95.004Å b: 95.004Å c: 93.714Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.233
Expression system: Spodoptera frugiperda

Quick links

Citations

49 review citations

Cognitive dysfunction in psychiatric disorders: characteristics, causes and the quest for improved therapy.
Millan et al. (2012)
48 more

6 mentions without citation

Phosphodiesterase-4 (PDE4) molecular pharmacology and Alzheimer's disease.
Gurney et al. (2015)
5 more