› 3g5g

X-ray diffraction
2.8Å resolution

Crystal Structure of the Wild-Type Restriction-Modification Controller Protein C.Esp1396I

Released:
DOI: 10.2210/pdb3g5g/pdb
PDB entry 3g5g coloured by chain, front view.
PDB entry 3g5g coloured by chain, side view.
PDB entry 3g5g coloured by chain, top view.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Structure of the restriction-modification controller protein C.Esp1396I.
Ball N, Streeter SD, Kneale GG, McGeehan JE
Acta Crystallogr D Biol Crystallogr 65 900-5 (2009)
PMID: 19690367

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 99 amino acids
Theoretical weight: 11.41 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P65
Unit cell:
a: 128.717Å b: 128.717Å c: 137.508Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.237 0.269
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems.
Ishikawa et al. (2010)
6 other articles

5 mentions without citation

Structural analysis of a novel class of R-M controller proteins: C.Csp231I from Citrobacter sp. RFL231.
McGeehan et al. (2011)
4 more