› 3g5y

X-ray diffraction
1.59Å resolution

Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR

Released:
DOI: 10.2210/pdb3g5y/pdb
PDB entry 3g5y coloured by chain, front view.
PDB entry 3g5y coloured by chain, side view.
PDB entry 3g5y coloured by chain, top view.
Source organisms:
Primary publication:
Antibodies specifically targeting a locally misfolded region of tumor associated EGFR.
Garrett TP, Burgess AW, Gan HK, Luwor RB, Cartwright G, Walker F, Orchard SG, Clayton AH, Nice EC, Rothacker J, Catimel B, Cavenee WK, Old LJ, Stockert E, Ritter G, Adams TE, Hoyne PA, Wittrup D, Chao G, Cochran JR, Luo C, Lou M, Huyton T, Xu Y, Fairlie WD, Yao S, Scott AM, Johns TG
Proc Natl Acad Sci U S A 106 5082-7 (2009)
PMID: 19289842

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208215 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
175 light chain Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
175 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 216 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Epidermal growth factor receptor Chain: E
Molecule details ›
Chain: E
Length: 16 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00533 (Residues: 311-326; Coverage: 1%)
Gene names: EGFR, ERBB, ERBB1, HER1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21212
Unit cell:
a: 83.176Å b: 69.267Å c: 71.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.25
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

11 review citations

Targeting the ERBB family in cancer: couples therapy.
Tebbutt et al. (2013)
10 more

2 mentions without citation

Antibodies specifically targeting a locally misfolded region of tumor associated EGFR.
Garrett et al. (2009)
1 more