› 3g8t

X-ray diffraction
3Å resolution

Crystal structure of the G33A mutant Bacillus anthracis glmS ribozyme bound to GlcN6P

Released:
DOI: 10.2210/pdb3g8t/pdb
PDB entry 3g8t coloured by chain, front view.
PDB entry 3g8t coloured by chain, side view.
PDB entry 3g8t coloured by chain, top view.
Source organisms:
Primary publication:
Structural and chemical basis for glucosamine 6-phosphate binding and activation of the glmS ribozyme.
Cochrane JC, Lipchock SV, Smith KD, Strobel SA
Biochemistry 48 3239-46 (2009)
PMID: 19228039

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140484 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*AP*(A2M)P*GP*CP*GP*CP*CP*AP*GP*AP*AP*CP*U)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 nucleotides
Theoretical weight: 4.18 KDa
Source organism: synthetic construct
Expression system: Not provided
glmS glucosamine-6-phosphate activated ribozyme Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 141 nucleotides
Theoretical weight: 45.61 KDa
Source organism: synthetic construct
Expression system: Not provided
Sequence domains: glmS glucosamine-6-phosphate activated ribozyme

Ligands and Environments

2 bound ligands:
Ligand GLP 4 x GLP
Ligand MG 10 x MG
2 modified residues:
Ligand A2M 4 x A2M
Ligand GTP 4 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 48.389Å b: 232.671Å c: 106.576Å
α: 90° β: 92.21° γ: 90°
R-values:
R R work R free
0.253 0.25 0.318
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Riboswitches and the RNA world.
Breaker RR. (2012)
13 more

4 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
3 more