› 3gb9

X-ray diffraction
2.3Å resolution

Human purine nucleoside phosphorylase double mutant E201Q,N243D complexed with 2-fluoroadenine

Released:
DOI: 10.2210/pdb3gb9/pdb
PDB entry 3gb9 coloured by chain, front view.
PDB entry 3gb9 coloured by chain, side view.
PDB entry 3gb9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a mutant human purine nucleoside phosphorylase with the prodrug, 2-fluoro-2'-deoxyadenosine and the cytotoxic drug, 2-fluoroadenine.
Afshar S, Sawaya MR, Morrison SL
Protein Sci 18 1107-14 (2009)
PMID: 19388075

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 34.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand A2F 3 x A2F
Ligand SO4 33 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 71.791Å b: 130.648Å c: 149.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Serum proteomics in biomedical research: a systematic review.
Zhang et al. (2013)
2 more

1 mention without citation

Structure of a mutant human purine nucleoside phosphorylase with the prodrug, 2-fluoro-2'-deoxyadenosine and the cytotoxic drug, 2-fluoroadenine.
Afshar et al. (2009)