› 3gct

X-ray diffraction
1.6Å resolution

STRUCTURE OF GAMMA-*CHYMOTRYPSIN IN THE RANGE $P*H 2.0 TO $P*H 10.5 SUGGESTS THAT GAMMA-CHYMOTRYPSIN IS A COVALENT ACYL-ENZYME ADDUCT AT LOW $P*H

Released:
DOI: 10.2210/pdb3gct/pdb
PDB entry 3gct coloured by chain, front view.
PDB entry 3gct coloured by chain, side view.
PDB entry 3gct coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of gamma-chymotrypsin in the range pH 2.0 to pH 10.5 suggests that gamma-chymotrypsin is a covalent acyl-enzyme adduct at low pH.
Dixon MM, Brennan RG, Matthews BW
Int J Biol Macromol 13 89-96 (1991)
PMID: 1888717

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133412 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Chymotrypsin A chain A Chain: E
Molecule details ›
Chain: E
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 1-13; Coverage: 5%)
Chymotrypsin A chain B Chain: F
Molecule details ›
Chain: F
Length: 131 amino acids
Theoretical weight: 13.93 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 16-146; Coverage: 54%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Chymotrypsin A chain C Chain: G
Molecule details ›
Chain: G
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 149-245; Coverage: 40%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
UNK PRO GLY ALA TYR PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 492 Da

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42212
Unit cell:
a: 70Å b: 70Å c: 97.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 not available

Quick links

Citations

2 review citations

Intracellular pH sensors: design principles and functional significance.
Srivastava et al. (2007)
1 more

1 mention without citation

Conversion of raltegravir carrying a 1,3,4-oxadiazole ring to a hydrolysis product upon pH changes decreases its antiviral activity.
Nakamura et al. (2024)