› 3gft

X-ray diffraction
2.27Å resolution

Human K-Ras (Q61H) in complex with a GTP analogue

Released:
DOI: 10.2210/pdb3gft/pdb
PDB entry 3gft coloured by chain, front view.
PDB entry 3gft coloured by chain, side view.
PDB entry 3gft coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tong Y, Tempel W, Shen L, Arrowsmith CH, Edwards AM, Sundstrom M, Weigelt J, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 187 amino acids
Theoretical weight: 21.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 6 x MG
Ligand GNP 6 x GNP
Ligand UNX 1 x UNX
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 63.535Å b: 118.275Å c: 156.836Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.267
Expression system: Escherichia coli

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