› 3gg0

X-ray diffraction
2.55Å resolution

Klebsiella pneumoniae BlrP1 pH 9.0 manganese/cy-diGMP complex

Released:
DOI: 10.2210/pdb3gg0/pdb
PDB entry 3gg0 coloured by chain, front view.
PDB entry 3gg0 coloured by chain, side view.
PDB entry 3gg0 coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Primary publication:
Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase.
Barends TR, Hartmann E, Griese JJ, Beitlich T, Kirienko NV, Ryjenkov DA, Reinstein J, Shoeman RL, Gomelsky M, Schlichting I
Nature 459 1015-8 (2009)
PMID: 19536266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 45.93 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli
UniProt:
  • Canonical: A6T8V8 (Residues: 1-405; Coverage: 100%)
Gene name: KPN_01598
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand C2E 2 x C2E
Ligand MN 4 x MN
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 69.02Å b: 96.89Å c: 126.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.295
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
49 more

6 mentions without citation

Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.
Krasteva et al. (2012)
5 more