› 3gi9

X-ray diffraction
2.48Å resolution

Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment

Released:
DOI: 10.2210/pdb3gi9/pdb
PDB entry 3gi9 coloured by chain, front view.
PDB entry 3gi9 coloured by chain, side view.
PDB entry 3gi9 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and mechanism of a Na+-independent amino acid transporter.
Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E
Science 325 1010-4 (2009)
PMID: 19608859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212286 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
7F11 Anti-ApcT Monoclonal Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.28 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
7F11 Anti-ApcT Monoclonal Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.98 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Uncharacterized protein MJ0609 Chain: C
Molecule details ›
Chain: C
Length: 444 amino acids
Theoretical weight: 48.33 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58026 (Residues: 1-435; Coverage: 100%)
Gene name: MJ0609
Sequence domains: Amino acid permease
Structure domains: Amino acid/polyamine transporter I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 148.365Å b: 45.9Å c: 158.088Å
α: 90° β: 97.75° γ: 90°
R-values:
R R work R free
0.25 0.248 0.296
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

43 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
42 more

11 mentions without citation

Crystallization chaperone strategies for membrane proteins.
Lieberman et al. (2011)
10 more