› 3giz

X-ray diffraction
2.2Å resolution

Crystal structure of the Fab fragment of anti-CD20 antibody Ofatumumab

Released:
DOI: 10.2210/pdb3giz/pdb
PDB entry 3giz coloured by chain, front view.
PDB entry 3giz coloured by chain, side view.
PDB entry 3giz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Fab fragment of therapeutic antibody Ofatumumab provides insights into the recognition mechanism with CD20.
Du J, Yang H, Guo Y, Ding J
Mol Immunol 46 2419-23 (2009)
PMID: 19427037

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234864 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab fragment of anti-CD20 antibody Ofatumumab, light chain Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab fragment of anti-CD20 antibody Ofatumumab, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 136.33Å b: 55.944Å c: 79.78Å
α: 90° β: 122.17° γ: 90°
R-values:
R R work R free
0.198 0.195 0.25
Expression system: Cricetulus griseus

Quick links

Citations

17 review citations

CD20 as a target for therapeutic type I and II monoclonal antibodies.
Beers et al. (2010)
16 more

11 mentions without citation

Structural basis of hepatitis C virus neutralization by broadly neutralizing antibody HCV1.
Kong et al. (2012)
10 more