› 3gnr

X-ray diffraction
1.81Å resolution

Crystal Structure of a Rice Os3BGlu6 Beta-Glucosidase with covalently bound 2-deoxy-2-fluoroglucoside to the catalytic nucleophile E396

Released:
DOI: 10.2210/pdb3gnr/pdb
PDB entry 3gnr coloured by chain, front view.
PDB entry 3gnr coloured by chain, side view.
PDB entry 3gnr coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides.
Seshadri S, Akiyama T, Opassiri R, Kuaprasert B, Cairns JK
Plant Physiol 151 47-58 (2009)
PMID: 19587102

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase 6 Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 55.32 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L7J2 (Residues: 38-521; Coverage: 100%)
Gene names: BGLU6, LOC_Os03g11420, Os03g0212800
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand G2F 1 x G2F
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 56.679Å b: 90.591Å c: 101.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.185
Expression system: Escherichia coli

Quick links

Citations

3 review citations

β-Glucosidases.
Ketudat Cairns et al. (2010)
2 more

1 mention without citation

Biochemical and structural characterization of a thermostable β-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.
Hassan et al. (2015)