› 3grf

X-ray diffraction
2Å resolution

X-ray Structure of Ornithine Transcarbamoylase from Giardia lamblia

Released:
DOI: 10.2210/pdb3grf/pdb
PDB entry 3grf coloured by chain, front view.
PDB entry 3grf coloured by chain, side view.
PDB entry 3grf coloured by chain, top view.
Source organism: Giardia intestinalis
Primary publication:
X-ray structure and kinetic properties of ornithine transcarbamoylase from the human parasite Giardia lamblia.
Galkin A, Kulakova L, Wu R, Gong M, Dunaway-Mariano D, Herzberg O
Proteins 76 1049-53 (2009)
PMID: 19507245

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ornithine carbamoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 36.55 KDa
Source organism: Giardia intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O76458 (Residues: 1-327; Coverage: 100%)
Gene name: OTC
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P63
Unit cell:
a: 95.49Å b: 95.49Å c: 56.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Sources and Fates of Carbamyl Phosphate: A Labile Energy-Rich Molecule with Multiple Facets.
Shi et al. (2018)
1 more

5 mentions without citation

Giardia duodenalis Virulence - "To Be, or Not To Be".
Argüello-García et al. (2021)
4 more