› 3gsn

X-ray diffraction
2.8Å resolution

Crystal structure of the public RA14 TCR in complex with the HCMV dominant NLV/HLA-A2 epitope

Released:
DOI: 10.2210/pdb3gsn/pdb
PDB entry 3gsn coloured by chain, front view.
PDB entry 3gsn coloured by chain, side view.
PDB entry 3gsn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural bases for the affinity-driven selection of a public TCR against a dominant human cytomegalovirus epitope.
Gras S, Saulquin X, Reiser JB, Debeaupuis E, Echasserieau K, Kissenpfennig A, Legoux F, Chouquet A, Le Gorrec M, Machillot P, Neveu B, Thielens N, Malissen B, Bonneville M, Housset D
J Immunol 183 430-7 (2009)
PMID: 19542454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208586 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: H
Molecule details ›
Chain: H
Length: 274 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
HCMV pp65 fragment 495-503 (NLVPMVATV) Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 943 Da
Beta-2-microglobulin Chain: L
Molecule details ›
Chain: L
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha chain constant Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
RA14 TCR beta chain (TRBV6-5, TRBD1, TRBJ1-2) Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 75.45Å b: 77.06Å c: 87.22Å
α: 90° β: 101.95° γ: 90°
R-values:
R R work R free
0.237 0.215 0.29
Expression system: Escherichia coli

Quick links

Citations

6 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
5 more

25 mentions without citation

Emerging Concepts in TCR Specificity: Rationalizing and (Maybe) Predicting Outcomes.
Singh et al. (2017)
24 more