› 3gt8

X-ray diffraction
2.96Å resolution

Crystal structure of the inactive EGFR kinase domain in complex with AMP-PNP

Released:
DOI: 10.2210/pdb3gt8/pdb
PDB entry 3gt8 coloured by chain, front view.
PDB entry 3gt8 coloured by chain, side view.
PDB entry 3gt8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism for activation of the EGF receptor catalytic domain by the juxtamembrane segment.
Jura N, Endres NF, Engel K, Deindl S, Das R, Lamers MH, Wemmer DE, Zhang X, Kuriyan J
Cell 137 1293-307 (2009)
PMID: 19563760

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132706 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 696-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Unknown peptide Chain: X
Molecule details ›
Chain: X
Length: 11 amino acids
Theoretical weight: 954 Da
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 61.764Å b: 72.411Å c: 143.376Å
α: 90° β: 101.74° γ: 90°
R-values:
R R work R free
0.219 0.216 0.275
Expression system: Spodoptera frugiperda

Quick links

Citations

87 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
86 more

28 mentions without citation

Catalytic control in the EGF receptor and its connection to general kinase regulatory mechanisms.
Jura et al. (2011)
27 more