› 3h2x

X-ray diffraction
2.2Å resolution

Crystal Structure of The Human Lymphoid Tyrosine Phosphatase Catalytic Domain

Released:
DOI: 10.2210/pdb3h2x/pdb
PDB entry 3h2x coloured by chain, front view.
PDB entry 3h2x coloured by chain, side view.
PDB entry 3h2x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human lymphoid tyrosine phosphatase catalytic domain: insights into redox regulation .
Tsai SJ, Sen U, Zhao L, Greenleaf WB, Dasgupta J, Fiorillo E, Orrú V, Bottini N, Chen XS
Biochemistry 48 4838-45 (2009)
PMID: 19371084

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 22 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 35.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y2R2 (Residues: 1-302; Coverage: 37%)
Gene names: PTPN22, PTPN8
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 42.495Å b: 68.868Å c: 58.701Å
α: 90° β: 101.57° γ: 90°
R-values:
R R work R free
0.178 0.176 0.204
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Regulation of protein tyrosine phosphatases by reversible oxidation.
Ostman et al. (2011)
17 more

6 mentions without citation

Crystal structure of the human lymphoid tyrosine phosphatase catalytic domain: insights into redox regulation .
Tsai et al. (2009)
5 more