› 3h3b

X-ray diffraction
2.45Å resolution

Crystal structure of the single-chain Fv (scFv) fragment of an anti-ErbB2 antibody chA21 in complex with residues 1-192 of ErbB2 extracellular domain

Released:
DOI: 10.2210/pdb3h3b/pdb
PDB entry 3h3b coloured by chain, front view.
PDB entry 3h3b coloured by chain, side view.
PDB entry 3h3b coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the down-regulation of overexpressed p185(her2/neu) protein of transformed cells by the antibody chA21.
Zhou H, Zha Z, Liu Y, Zhang H, Zhu J, Hu S, Shen G, Cheng L, Niu L, Greene MI, Teng M, Liu J
J Biol Chem 286 31676-83 (2011)
PMID: 21680730

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233923 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor tyrosine-protein kinase erbB-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04626 (Residues: 23-214; Coverage: 16%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains: Receptor L domain
Structure domains: Receptor L-domain
anti-ErbB2 antibody chA21 Chains: C, D
Molecule details ›
Chains: C, D
Length: 259 amino acids
Theoretical weight: 27.56 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P212121
Unit cell:
a: 82.177Å b: 87.2Å c: 108.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.255
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

2 review citations

Tumor-targeting domains for chimeric antigen receptor T cells.
Bezverbnaya et al. (2017)
1 more

11 mentions without citation

Designer Oncolytic Adenovirus: Coming of Age.
Baker et al. (2018)
10 more