› 3h3p

X-ray diffraction
2.7Å resolution

Crystal structure of HIV epitope-scaffold 4E10 Fv complex

Released:
DOI: 10.2210/pdb3h3p/pdb
PDB entry 3h3p coloured by chain, front view.
PDB entry 3h3p coloured by chain, side view.
PDB entry 3h3p coloured by chain, top view.
Source organisms:
Primary publication:
Interactions between lipids and human anti-HIV antibody 4E10 can be reduced without ablating neutralizing activity.
Xu H, Song L, Kim M, Holmes MA, Kraft Z, Sellhorn G, Reinherz EL, Stamatatos L, Strong RK
J Virol 84 1076-88 (2010)
PMID: 19906921

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211625 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fv 4E10 heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 137 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fv 4E10 light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 114 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
4E10_S0_1TJLC_004_N Chains: S, T
Molecule details ›
Chains: S, T
Length: 85 amino acids
Theoretical weight: 10.5 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 92.4Å b: 92.4Å c: 272.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.287
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Rational antibody-based HIV-1 vaccine design: current approaches and future directions.
Walker et al. (2010)
5 more

3 mentions without citation

Max Bergmann lecture protein epitope mimetics in the age of structural vaccinology.
Robinson JA. (2013)
2 more