› 3h77

X-ray diffraction
1.8Å resolution

Crystal structure of Pseudomonas aeruginosa PqsD in a covalent complex with anthranilate

Released:
DOI: 10.2210/pdb3h77/pdb
PDB entry 3h77 coloured by chain, front view.
PDB entry 3h77 coloured by chain, side view.
PDB entry 3h77 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure of PqsD, a Pseudomonas quinolone signal biosynthetic enzyme, in complex with anthranilate.
Bera AK, Atanasova V, Robinson H, Eisenstein E, Coleman JP, Pesci EC, Parsons JF
Biochemistry 48 8644-55 (2009)
PMID: 19694421

Function and Biology Details

Reaction catalysed: 
(1a) anthraniloyl-CoA + L-cysteinyl-[PqsD protein] = S-anthraniloyl-L-cysteinyl-[PqsD protein] + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthraniloyl-CoA anthraniloyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 38.86 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P20582 (Residues: 2-337; Coverage: 100%)
Gene names: PA0999, pqsD
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand COW 2 x COW
1 modified residue:
Ligand CSJ 2 x CSJ

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 67.894Å b: 59.656Å c: 83.427Å
α: 90° β: 103.95° γ: 90°
R-values:
R R work R free
0.154 0.152 0.195
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Quinolones: from antibiotics to autoinducers.
Heeb et al. (2011)
7 more

6 mentions without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)
5 more