› 3ham

X-ray diffraction
2.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 35.4 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EVD7 (Residues: 1-299; Coverage: 100%)
Gene name: aph(2')-Ib
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LLL 2 x LLL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 79.7Å b: 58.8Å c: 81.4Å
α: 90° β: 98.4° γ: 90°
R-values:
R R work R free
0.213 0.21 0.271
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The future of aminoglycosides: the end or renaissance?
Houghton et al. (2010)
4 more

10 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
9 more