› 3hb6

X-ray diffraction
2.3Å resolution

Inactive mutant H54F of Proteus mirabilis catalase

Released:
DOI: 10.2210/pdb3hb6/pdb
PDB entry 3hb6 coloured by chain, front view.
PDB entry 3hb6 coloured by chain, side view.
PDB entry 3hb6 coloured by chain, top view.
Source organism: Proteus mirabilis
Primary publication:
Verdoheme formation in Proteus mirabilis catalase.
Andreoletti P, Mouesca JM, Gouet P, Jaquinod M, Capeillère-Blandin C, Jouve HM
Biochim Biophys Acta 1790 741-53 (2009)
PMID: 19394409

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 55.7 KDa
Source organism: Proteus mirabilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42321 (Residues: 1-484; Coverage: 100%)
Gene name: katA
Sequence domains:
Structure domains: Catalase core domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6222
Unit cell:
a: 109.3Å b: 109.3Å c: 249.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Mechanisms of oxidant generation by catalase.
Heck et al. (2010)
1 more