› 3hbx

X-ray diffraction
2.67Å resolution

Crystal structure of GAD1 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb3hbx/pdb
PDB entry 3hbx coloured by chain, front view.
PDB entry 3hbx coloured by chain, side view.
PDB entry 3hbx coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
A common structural basis for pH- and calmodulin-mediated regulation in plant glutamate decarboxylase.
Gut H, Dominici P, Pilati S, Astegno A, Petoukhov MV, Svergun DI, Grütter MG, Capitani G
J Mol Biol 392 334-51 (2009)
PMID: 19580813

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 502 amino acids
Theoretical weight: 57.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q42521 (Residues: 1-502; Coverage: 100%)
Gene names: At5g17330, GAD, GAD1, GDH1, MKP11.30, MKP11_18
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 6 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P32
Unit cell:
a: 118.098Å b: 118.098Å c: 200.576Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.221
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Structural characterization of proteins and complexes using small-angle X-ray solution scattering.
Mertens et al. (2010)
9 more

4 mentions without citation

Automated evaluation of quaternary structures from protein crystals.
Bliven et al. (2018)
3 more