› 3hd7

X-ray diffraction
3.4Å resolution

HELICAL EXTENSION OF THE NEURONAL SNARE COMPLEX INTO THE MEMBRANE, spacegroup C 1 2 1

Released:
DOI: 10.2210/pdb3hd7/pdb
PDB entry 3hd7 coloured by chain, front view.
PDB entry 3hd7 coloured by chain, side view.
PDB entry 3hd7 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Helical extension of the neuronal SNARE complex into the membrane.
Stein A, Weber G, Wahl MC, Jahn R
Nature 460 525-8 (2009)
PMID: 19571812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152474 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 91 amino acids
Theoretical weight: 10.49 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 30-116; Coverage: 75%)
Gene names: Syb2, Vamp2
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chains: B, F
Molecule details ›
Chains: B, F
Length: 109 amino acids
Theoretical weight: 12.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 183-288; Coverage: 37%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: C, G
Molecule details ›
Chains: C, G
Length: 80 amino acids
Theoretical weight: 9.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 7-83; Coverage: 37%)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: D, H
Molecule details ›
Chains: D, H
Length: 68 amino acids
Theoretical weight: 7.75 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 141-204; Coverage: 31%)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand GGG 3 x GGG
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 265.412Å b: 135.49Å c: 58.689Å
α: 90° β: 96.34° γ: 90°
R-values:
R R work R free
0.241 0.239 0.271
Expression system: Escherichia coli

Quick links

Citations

62 review citations

The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
Rizo et al. (2012)
61 more

27 mentions without citation

Chaperoning SNARE Folding and Assembly.
Zhang et al. (2021)
26 more