› 3hf9

X-ray diffraction
2.88Å resolution

Crystal Structure of Mycobacterium Tuberculosis Proteasome open-gate mutant modified by inhibitor GL1

Released:
DOI: 10.2210/pdb3hf9/pdb
PDB entry 3hf9 coloured by chain, front view.
PDB entry 3hf9 coloured by chain, side view.
PDB entry 3hf9 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Inhibitors selective for mycobacterial versus human proteasomes.
Lin G, Li D, de Carvalho LP, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H, Nathan C
Nature 461 621-6 (2009)
PMID: 19759536

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161590 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome subunit alpha Chains: 1, 3, A, B, D, F, I, K, M, O, Q, S, U, W, Y, a, b, d, f, i, k, m, o, q, s, u, w, y
Molecule details ›
Chains: 1, 3, A, B, D, F, I, K, M, O, Q, S, U, W, Y, a, b, d, f, i, k, m, o, q, s, u, w, y
Length: 240 amino acids
Theoretical weight: 25.97 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 10-248; Coverage: 96%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha Chains: 2, 4, C, E, G, H, J, L, N, P, R, T, V, X, Z, c, e, g, h, j, l, n, p, r, t, v, x, z
Molecule details ›
Chains: 2, 4, C, E, G, H, J, L, N, P, R, T, V, X, Z, c, e, g, h, j, l, n, p, r, t, v, x, z
Length: 240 amino acids
Theoretical weight: 25.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 29-54, 55-88, 89-131, 132-139, 140-141, 142-144, 145-203, 204-216; Coverage: 73%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 119.583Å b: 209.615Å c: 284.933Å
α: 90° β: 102.01° γ: 90°
R-values:
R R work R free
0.22 0.217 0.266
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Ubiquitin-like protein conjugation and the ubiquitin-proteasome system as drug targets.
Bedford et al. (2011)
49 more

3 mentions without citation

Inhibitors selective for mycobacterial versus human proteasomes.
Lin et al. (2009)
2 more