› 3hgb

X-ray diffraction
1.75Å resolution

Crystal structure of glycine cleavage system protein H from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3hgb/pdb
PDB entry 3hgb coloured by chain, front view.
PDB entry 3hgb coloured by chain, side view.
PDB entry 3hgb coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
Abendroth J, McCormick MS, Edwards TE, Staker B, Loewen R, Gifford M, Rifkin J, Mayer C, Guo W, Zhang Y, Myler P, Kelley A, Analau E, Hewitt SN, Napuli AJ, Kuhn P, Ruth RD, Stewart LJ
J Struct Funct Genomics 11 91-100 (2010)
PMID: 20364333

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine cleavage system H protein Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 16.54 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN55 (Residues: 1-134; Coverage: 100%)
Gene names: MTCY1A11.17c, Rv1826, gcvH
Sequence domains: Glycine cleavage H-protein
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 86.86Å b: 51.47Å c: 32.53Å
α: 90° β: 94.64° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli

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Citations

6 citation in other articles

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Eggl et al. (2016)
5 more

4 mentions without citation

Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens.
Rack et al. (2015)
3 more