› 3hgk

X-ray diffraction
3.3Å resolution

crystal structure of effect protein AvrptoB complexed with kinase Pto

Released:
DOI: 10.2210/pdb3hgk/pdb
PDB entry 3hgk coloured by chain, front view.
PDB entry 3hgk coloured by chain, side view.
PDB entry 3hgk coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the complex between Pseudomonas effector AvrPtoB and the tomato Pto kinase reveals both a shared and a unique interface compared with AvrPto-Pto.
Dong J, Xiao F, Fan F, Gu L, Cang H, Martin GB, Chai J
Plant Cell 21 1846-59 (2009)
PMID: 19509331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174925 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 37.33 KDa
Source organism: Solanum pimpinellifolium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q40234 (Residues: 1-321; Coverage: 100%)
Gene name: Pto
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Effector protein HopAB2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 85 amino acids
Theoretical weight: 9.43 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RSY1 (Residues: 121-205; Coverage: 15%)
Gene names: PSPTO_3087, avrPtoB, hopAB2
Structure domains: Monooxygenase

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 61.07Å b: 104.47Å c: 298.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.318 0.317 0.33
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Receptor-Like Cytoplasmic Kinases: Central Players in Plant Receptor Kinase-Mediated Signaling.
Liang et al. (2018)
14 more

9 mentions without citation

A molecular roadmap to the plant immune system.
Bentham et al. (2020)
8 more