› 3hje

X-ray diffraction
1.9Å resolution

Crystal structure of sulfolobus tokodaii hypothetical maltooligosyl trehalose synthase

Released:
DOI: 10.2210/pdb3hje/pdb
PDB entry 3hje coloured by chain, front view.
PDB entry 3hje coloured by chain, side view.
PDB entry 3hje coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
Structure of ST0929, a putative glycosyl transferase from Sulfolobus tokodaii.
Cielo CB, Okazaki S, Suzuki A, Mizushima T, Masui R, Kuramitsu S, Yamane T
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 397-400 (2010)
PMID: 20383007

Function and Biology Details

Reaction catalysed: 
4-((1->4)-alpha-D-glucosyl)(n-1)-D-glucose = 1-alpha-D-((1->4)-alpha-D-glucosyl)(n-1)-alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 704 amino acids
Theoretical weight: 83.73 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q973H2 (Residues: 1-704; Coverage: 100%)
Gene names: ST0929, STK_09290, mts, treY
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 63.992Å b: 86.1Å c: 70.13Å
α: 90° β: 95.43° γ: 90°
R-values:
R R work R free
0.172 0.171 0.206
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis.
Zhang et al. (2011)
1 more

2 mentions without citation

Structure of ST0929, a putative glycosyl transferase from Sulfolobus tokodaii.
Cielo et al. (2010)
1 more