› 3hn7

X-ray diffraction
1.65Å resolution

Crystal structure of a murein peptide ligase mpl (psyc_0032) from psychrobacter arcticus 273-4 at 1.65 A resolution

Released:
DOI: 10.2210/pdb3hn7/pdb
PDB entry 3hn7 coloured by chain, front view.
PDB entry 3hn7 coloured by chain, side view.
PDB entry 3hn7 coloured by chain, top view.
Source organism: Psychrobacter arcticus 273-4
Primary publication:
Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein.
Das D, Hervé M, Feuerhelm J, Farr CL, Chiu HJ, Elsliger MA, Knuth MW, Klock HE, Miller MD, Godzik A, Lesley SA, Deacon AM, Mengin-Lecreulx D, Wilson IA
PLoS One 6 e17624 (2011)
PMID: 21445265

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase Chain: A
Molecule details ›
Chain: A
Length: 524 amino acids
Theoretical weight: 58.01 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4FVQ2 (Residues: 1-505; Coverage: 100%)
Gene names: Psyc_0032, mpl
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 105.452Å b: 52.755Å c: 89.986Å
α: 90° β: 98.47° γ: 90°
R-values:
R R work R free
0.156 0.154 0.187
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacterial cell-wall recycling.
Johnson et al. (2013)
6 more

2 mentions without citation

Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp.
Robinson et al. (2010)
1 more