Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
Restriction endonuclease type IIe and DNA (preferred)
PDBe Complex ID:
PDB-CPX-116374 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (3 distinct):
Restriction endonuclease type IIe
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 19.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains: Restriction endonuclease EcoRII, N-terminal
Structure domains: DNA-binding pseudobarrel domain
Length: 181 amino acids
Theoretical weight: 19.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
O69414 (Residues: 2-170; Coverage: 42%)
Sequence domains: Restriction endonuclease EcoRII, N-terminal
Structure domains: DNA-binding pseudobarrel domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup:
P43212
Expression systems:
- Escherichia coli
- Not provided
