› 3hqj

X-ray diffraction
1.95Å resolution

Structure-function analysis of Mycobacterium tuberculosis acyl carrier protein synthase (AcpS).

Released:
DOI: 10.2210/pdb3hqj/pdb
PDB entry 3hqj coloured by chain, front view.
PDB entry 3hqj coloured by chain, side view.
PDB entry 3hqj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure-function analysis of the acyl carrier protein synthase (AcpS) from Mycobacterium tuberculosis.
Dym O, Albeck S, Peleg Y, Schwarz A, Shakked Z, Burstein Y, Zimhony O
J Mol Biol 393 937-50 (2009)
PMID: 19733180

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holo-[acyl-carrier-protein] synthase Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQD3 (Residues: 1-130; Coverage: 100%)
Gene names: MTV009.08c, Rv2523c, acpS
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P23
Unit cell:
a: 77.443Å b: 77.443Å c: 77.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.252
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life.
Beld et al. (2014)
2 more

3 mentions without citation

Structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria.
Halavaty et al. (2012)
2 more