› 3hw2

X-ray diffraction
3.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176318 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Secreted effector protein SifA Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q56061 (Residues: 1-336; Coverage: 100%)
Gene names: STM1224, sifA
Sequence domains: Sif protein
Structure domains:
Pleckstrin homology domain-containing family M member 2 Chain: B
Molecule details ›
Chain: B
Length: 105 amino acids
Theoretical weight: 11.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IWE5 (Residues: 771-875; Coverage: 10%)
Gene names: KIAA0842, PLEKHM2, SKIP
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 91.799Å b: 110.866Å c: 44.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.24 0.309
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Functions of the Salmonella pathogenicity island 2 (SPI-2) type III secretion system effectors.
Figueira et al. (2012)
10 more

2 mentions without citation

Mimicking GEFs: a common theme for bacterial pathogens.
Orchard et al. (2012)
1 more