› 3hz3

X-ray diffraction
2.22Å resolution

Lactobacillus reuteri N-terminally truncated glucansucrase GTF180(D1025N)-sucrose complex

Released:
DOI: 10.2210/pdb3hz3/pdb
PDB entry 3hz3 coloured by chain, front view.
PDB entry 3hz3 coloured by chain, side view.
PDB entry 3hz3 coloured by chain, top view.
Source organism: Limosilactobacillus reuteri
Primary publication:
Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes.
Vujicic-Zagar A, Pijning T, Kralj S, López CA, Eeuwema W, Dijkhuizen L, Dijkstra BW
Proc Natl Acad Sci U S A 107 21406-11 (2010)
PMID: 21118988

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1039 amino acids
Theoretical weight: 116.73 KDa
Source organism: Limosilactobacillus reuteri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SBN3 (Residues: 742-1772; Coverage: 60%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P1
Unit cell:
a: 57.782Å b: 66.233Å c: 82.724Å
α: 107.13° β: 101.37° γ: 85.4°
R-values:
R R work R free
0.195 0.193 0.243
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid et al. (2015)
13 more

21 mentions without citation

Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.
Meng et al. (2016)
20 more