› 3hzt

X-ray diffraction
2Å resolution

Crystal structure of Toxoplasma gondii CDPK3, TGME49_105860

Released:
DOI: 10.2210/pdb3hzt/pdb
PDB entry 3hzt coloured by chain, front view.
PDB entry 3hzt coloured by chain, side view.
PDB entry 3hzt coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.
Wernimont AK, Artz JD, Finerty P, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R
Nat Struct Mol Biol 17 596-601 (2010)
PMID: 20436473

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-dependent protein kinase CDPK3 Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 53.43 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3HNM6 (Residues: 72-537; Coverage: 87%)
Gene name: TGRH88_017420
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GOL 8 x GOL
Ligand J60 1 x J60
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 71.051Å b: 43.761Å c: 84.821Å
α: 90° β: 96.95° γ: 90°
R-values:
R R work R free
0.21 0.207 0.252
Expression system: Escherichia coli

Quick links

Citations

35 review citations

CDPKs in immune and stress signaling.
Boudsocq et al. (2013)
34 more

7 mentions without citation

Designing selective inhibitors for calcium-dependent protein kinases in apicomplexans.
Hui et al. (2015)
6 more