› 3i4e

X-ray diffraction
2.69Å resolution

Crystal structure of Isocitrate Lyase from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3i4e/pdb
PDB entry 3i4e coloured by chain, front view.
PDB entry 3i4e coloured by chain, side view.
PDB entry 3i4e coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Entry authors: Staker BL, Arakaki T, Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 48.13 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63SY3 (Residues: 1-435; Coverage: 100%)
Gene names: BPSL2188, aceA, icl
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 77.666Å b: 137.025Å c: 160.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.277
Expression system: Escherichia coli

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