› 3i5r

X-ray diffraction
1.7Å resolution

PI3K SH3 domain in complex with a peptide ligand

Released:
DOI: 10.2210/pdb3i5r/pdb
PDB entry 3i5r coloured by chain, front view.
PDB entry 3i5r coloured by chain, side view.
PDB entry 3i5r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of the phosphatidylinositol 3-kinase (PI3K) SH3 domain in complex with a peptide ligand: role of the anchor residue in ligand binding.
Batra-Safferling R, Granzin J, Mödder S, Hoffmann S, Willbold D
Biol Chem 391 33-42 (2010)
PMID: 19919182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151137 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27986 (Residues: 1-83; Coverage: 12%)
Gene names: GRB1, PIK3R1
Structure domains: SH3 Domains
Peptide ligand Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 60.365Å b: 60.365Å c: 46.525Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Multiple roles for the p85α isoform in the regulation and function of PI3K signalling and receptor trafficking.
Mellor et al. (2012)
1 more

10 mentions without citation

Regulation of the PI3K pathway through a p85α monomer-homodimer equilibrium.
Cheung et al. (2015)
9 more