› 3i5y

X-ray diffraction
2.49Å resolution

Structure of Mss116p bound to ssRNA containing a single 5-BrU and AMP-PNP

Released:
DOI: 10.2210/pdb3i5y/pdb
PDB entry 3i5y coloured by chain, front view.
PDB entry 3i5y coloured by chain, side view.
PDB entry 3i5y coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA.
Del Campo M, Lambowitz AM
Mol Cell 35 598-609 (2009)
PMID: 19748356

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147352 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase MSS116, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 64.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15424 (Residues: 37-597; Coverage: 85%)
Gene names: MSS116, YD9346.05C, YDR194C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
5'-R(*UP*UP*UP*(5BU)P*UP*UP*UP*UP*UP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
1 modified residue:
Ligand 5BU 1 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 89.19Å b: 126.352Å c: 55.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.242
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
29 more

2 mentions without citation

Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA.
Del Campo et al. (2009)
1 more