› 3i6j

X-ray diffraction
1.3Å resolution

Ribonuclease A by Classical hanging drop method after high X-Ray dose on ESRF ID14-2 beamline

Released:
DOI: 10.2210/pdb3i6j/pdb
PDB entry 3i6j coloured by chain, front view.
PDB entry 3i6j coloured by chain, side view.
PDB entry 3i6j coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Pechkova E, Tripathi SK, Ravelli R, McSweeney S, Nicolini C

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3221
Unit cell:
a: 64.362Å b: 64.362Å c: 63.745Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.234

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