› 3i7z

X-ray diffraction
2.3Å resolution

Protein Tyrosine Phosphatase 1B - Transition state analog for the first catalytic step

Released:
DOI: 10.2210/pdb3i7z/pdb
PDB entry 3i7z coloured by chain, front view.
PDB entry 3i7z coloured by chain, side view.
PDB entry 3i7z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the reaction of protein-tyrosine phosphatase 1B: crystal structures for transition state analogs of both catalytic steps.
Brandão TA, Hengge AC, Johnson SJ
J Biol Chem 285 15874-83 (2010)
PMID: 20236928

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-148267 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
EGFR receptor fragment Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 725 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand VO4 1 x VO4
Ligand TRS 2 x TRS
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 88.02Å b: 88.02Å c: 118.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.233
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Vanadium in Biological Action: Chemical, Pharmacological Aspects, and Metabolic Implications in Diabetes Mellitus.
Treviño et al. (2019)
14 more

11 mentions without citation

The metal face of protein tyrosine phosphatase 1B.
Bellomo et al. (2016)
10 more