› 3igo

X-ray diffraction
2.25Å resolution

Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920

Released:
DOI: 10.2210/pdb3igo/pdb
PDB entry 3igo coloured by chain, front view.
PDB entry 3igo coloured by chain, side view.
PDB entry 3igo coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.
Wernimont AK, Artz JD, Finerty P, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R
Nat Struct Mol Biol 17 596-601 (2010)
PMID: 20436473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-domain protein kinase 1, putative Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 56.15 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: A3FQ16 (Residues: 70-538; Coverage: 87%)
Gene name: cgd3_920
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand ANP 1 x ANP
Ligand GOL 6 x GOL
Ligand SRT 1 x SRT
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.388Å b: 55.55Å c: 81.697Å
α: 90° β: 105.25° γ: 90°
R-values:
R R work R free
0.205 0.203 0.258
Expression system: Escherichia coli

Quick links

Citations

35 review citations

CDPKs in immune and stress signaling.
Boudsocq et al. (2013)
34 more

2 mentions without citation

Designing selective inhibitors for calcium-dependent protein kinases in apicomplexans.
Hui et al. (2015)
1 more