› 3ik7

X-ray diffraction
1.97Å resolution

Human glutathione transferase a4-4 with GSDHN

Released:
DOI: 10.2210/pdb3ik7/pdb
PDB entry 3ik7 coloured by chain, front view.
PDB entry 3ik7 coloured by chain, side view.
PDB entry 3ik7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate specificity combined with stereopromiscuity in glutathione transferase A4-4-dependent metabolism of 4-hydroxynonenal.
Balogh LM, Le Trong I, Kripps KA, Shireman LM, Stenkamp RE, Zhang W, Mannervik B, Atkins WM
Biochemistry 49 1541-8 (2010)
PMID: 20085333

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 25.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15217 (Residues: 1-222; Coverage: 100%)
Gene name: GSTA4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand BOB 4 x BOB
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 59.023Å b: 153.041Å c: 60.831Å
α: 90° β: 103.1° γ: 90°
R-values:
R R work R free
0.209 0.206 0.252
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Chemistry and biochemistry of lipid peroxidation products.
Guéraud et al. (2010)
8 more

4 mentions without citation

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
3 more