› 3iko

X-ray diffraction
3.2Å resolution

Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex

Released:
DOI: 10.2210/pdb3iko/pdb
PDB entry 3iko coloured by chain, front view.
PDB entry 3iko coloured by chain, side view.
PDB entry 3iko coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a trimeric nucleoporin complex reveals alternate oligomerization states.
Nagy V, Hsia KC, Debler EW, Kampmann M, Davenport AM, Blobel G, Hoelz A
Proc Natl Acad Sci U S A 106 17693-8 (2009)
PMID: 19805193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155989 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein SEC13 Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 297 amino acids
Theoretical weight: 33.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04491 (Residues: 1-297; Coverage: 100%)
Gene names: ANU3, L8167.4, SEC13, YLR208W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Nucleoporin NUP145C Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 442 amino acids
Theoretical weight: 51.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P49687 (Residues: 731-1158; Coverage: 33%)
Gene names: NUP145, RAT10, YGL092W
Sequence domains: Nuclear protein 96
Structure domains:
Nucleoporin NUP84 Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 460 amino acids
Theoretical weight: 52.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52891 (Residues: 1-460; Coverage: 63%)
Gene names: NUP84, YDL116W
Sequence domains: Nuclear pore protein 84 / 107
Structure domains: Hyaluronidase domain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 101.397Å b: 194.05Å c: 327.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.273
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The structure of the nuclear pore complex.
Hoelz et al. (2011)
13 more

11 mentions without citation

In situ structural analysis of the human nuclear pore complex.
von Appen et al. (2015)
10 more