› 3imx

X-ray diffraction
2Å resolution

Crystal Structure of human glucokinase in complex with a synthetic activator

Released:
DOI: 10.2210/pdb3imx/pdb
PDB entry 3imx coloured by chain, front view.
PDB entry 3imx coloured by chain, side view.
PDB entry 3imx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Investigation of functionally liver selective glucokinase activators for the treatment of type 2 diabetes.
Bebernitz GR, Beaulieu V, Dale BA, Deacon R, Duttaroy A, Gao J, Grondine MS, Gupta RC, Kakmak M, Kavana M, Kirman LC, Liang J, Maniara WM, Munshi S, Nadkarni SS, Schuster HF, Stams T, St Denny I, Taslimi PM, Vash B, Caplan SL
J Med Chem 52 6142-52 (2009)
PMID: 19746978

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-4 Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 50.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 16-465; Coverage: 97%)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand B84 1 x B84
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 45.5Å b: 90.3Å c: 116.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Liver glucokinase: An overview on the regulatory mechanisms of its activity.
Massa et al. (2011)
8 more

11 mentions without citation

Evaluation of the Anti-Diabetic Activity of Some Common Herbs and Spices: Providing New Insights with Inverse Virtual Screening.
Pereira et al. (2019)
10 more