› 3io0

X-ray diffraction
3Å resolution

Crystal structure of EtuB from Clostridium kluyveri

Released:
DOI: 10.2210/pdb3io0/pdb
PDB entry 3io0 coloured by chain, front view.
PDB entry 3io0 coloured by chain, side view.
PDB entry 3io0 coloured by chain, top view.
Source organism: Clostridium kluyveri DSM 555
Primary publication:
Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri.
Heldt D, Frank S, Seyedarabi A, Ladikis D, Parsons JB, Warren MJ, Pickersgill RW
Biochem J 423 199-207 (2009)
PMID: 19635047

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BMC circularly permuted domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 23.76 KDa
Source organism: Clostridium kluyveri DSM 555
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5N734 (Residues: 75-304; Coverage: 76%)
Gene name: CKL_1073
Sequence domains: BMC domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I213
Unit cell:
a: 173.41Å b: 173.41Å c: 173.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Bacterial microcompartments.
Kerfeld et al. (2010)
9 more

5 mentions without citation

The structure of CcmP, a tandem bacterial microcompartment domain protein from the β-carboxysome, forms a subcompartment within a microcompartment.
Cai et al. (2013)
4 more