› 3io3

X-ray diffraction
1.8Å resolution

GEt3 with ADP from D. Hansenii in Closed form

Released:
DOI: 10.2210/pdb3io3/pdb
PDB entry 3io3 coloured by chain, front view.
PDB entry 3io3 coloured by chain, side view.
PDB entry 3io3 coloured by chain, top view.
Source organism: Debaryomyces hansenii
Primary publication:
The crystal structures of yeast Get3 suggest a mechanism for tail-anchored protein membrane insertion.
Hu J, Li J, Qian X, Denic V, Sha B
PLoS One 4 e8061 (2009)
PMID: 19956640

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-179428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase GET3 Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.25 KDa
Source organism: Debaryomyces hansenii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6BSM0 (Residues: 1-348; Coverage: 100%)
Gene names: DEHA2D07832g, GET3
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand ADP 1 x ADP
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GOL 3 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I222
Unit cell:
a: 59.851Å b: 87.292Å c: 230.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
14 more

3 mentions without citation

The complex process of GETting tail-anchored membrane proteins to the ER.
Chartron et al. (2012)
2 more