› 3iol

X-ray diffraction
2.1Å resolution

Crystal structure of Glucagon-Like Peptide-1 in complex with the extracellular domain of the Glucagon-Like Peptide-1 Receptor

Released:
DOI: 10.2210/pdb3iol/pdb
PDB entry 3iol coloured by chain, front view.
PDB entry 3iol coloured by chain, side view.
PDB entry 3iol coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of glucagon-like peptide-1 in complex with the extracellular domain of the glucagon-like peptide-1 receptor.
Underwood CR, Garibay P, Knudsen LB, Hastrup S, Peters GH, Rudolph R, Reedtz-Runge S
J Biol Chem 285 723-30 (2010)
PMID: 19861722

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134156 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucagon-like peptide 1 receptor Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43220 (Residues: 24-145; Coverage: 28%)
Gene name: GLP1R
Sequence domains: Hormone receptor domain
Structure domains: GPCR, family 2, extracellular hormone receptor domain
Glucagon-like peptide 1(7-37) Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01275 (Residues: 98-128; Coverage: 19%)
Gene name: GCG
Sequence domains: Peptide hormone

Ligands and Environments

1 bound ligand:
Ligand 10M 1 x 10M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Unit cell:
a: 35.67Å b: 42.67Å c: 95.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

28 review citations

Glucagon-like peptide 1 (GLP-1).
Müller et al. (2019)
27 more

46 mentions without citation

Albumin-based drug delivery: harnessing nature to cure disease.
Larsen et al. (2016)
45 more