› 3iov

X-ray diffraction
3.7Å resolution

Huntingtin amino-terminal region with 17 Gln residues - crystal C99

Released:
DOI: 10.2210/pdb3iov/pdb
PDB entry 3iov coloured by chain, front view.
PDB entry 3iov coloured by chain, side view.
PDB entry 3iov coloured by chain, top view.
Source organisms:
Primary publication:
Secondary structure of Huntingtin amino-terminal region.
Kim MW, Chelliah Y, Kim SW, Otwinowski Z, Bezprozvanny I
Structure 17 1205-12 (2009)
PMID: 19748341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Huntingtin; Maltose/maltodextrin-binding periplasmic protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 449 amino acids
Theoretical weight: 49.32 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
  • Canonical: P42858 (Residues: 1-64; Coverage: 2%)
Gene names: HD, HTT, IT15, JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 163.82Å b: 101.19Å c: 134.82Å
α: 90° β: 99.23° γ: 90°
R-values:
R R work R free
0.265 0.264 0.28
Expression system: Escherichia coli BL21

Quick links

Citations

32 review citations

Huntington's disease: from molecular pathogenesis to clinical treatment.
Ross et al. (2011)
31 more

7 mentions without citation

Therapeutic approaches to preventing cell death in Huntington disease.
Kaplan et al. (2012)
6 more