› 3isq

X-ray diffraction
1.75Å resolution

Crystal structure of human 4-Hydroxyphenylpyruvate dioxygenase

Released:
DOI: 10.2210/pdb3isq/pdb
PDB entry 3isq coloured by chain, front view.
PDB entry 3isq coloured by chain, side view.
PDB entry 3isq coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pilka ES, Shafqat N, Cocking R, Bray JE, Krojer T, Pike ACW, von Delft F, Yue WW, Arrowsmith CH, Weigelt J, Edwards A, Bountra C, Oppermann U, Kavanagh KL, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
4-hydroxyphenylpyruvate + O(2) = homogentisate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyphenylpyruvate dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 45.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P32754 (Residues: 8-393; Coverage: 98%)
Gene names: HPD, PPD
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

4 bound ligands:
Ligand CO 1 x CO
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3121
Unit cell:
a: 99.206Å b: 99.206Å c: 87.726Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.145 0.144 0.174
Expression system: Escherichia coli

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