› 3itk

X-ray diffraction
2.4Å resolution

Crystal structure of human UDP-glucose dehydrogenase Thr131Ala, apo form.

Released:
DOI: 10.2210/pdb3itk/pdb
PDB entry 3itk coloured by chain, front view.
PDB entry 3itk coloured by chain, side view.
PDB entry 3itk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mechanism of human UDP-glucose 6-dehydrogenase.
Egger S, Chaikuad A, Kavanagh KL, Oppermann U, Nidetzky B
J Biol Chem 286 23877-87 (2011)
PMID: 21502315

Function and Biology Details

Reaction catalysed: 
UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 6-dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 467 amino acids
Theoretical weight: 52.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60701 (Residues: 1-466; Coverage: 94%)
Gene name: UGDH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PG4 5 x PG4
Ligand EDO 17 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 89.06Å b: 106.61Å c: 349.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.241
Expression system: Escherichia coli

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Citations

5 review citations

Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization.
Simeonov A. (2013)
4 more

10 mentions without citation

Roles of Prolyl Isomerases in RNA-Mediated Gene Expression.
Thapar R. (2015)
9 more